Composite

Part:BBa_K2650003:Experience

Designed by: Lisa Oberding   Group: iGEM18_OLS_Canmore_Canada   (2018-10-05)

Gene ID: BslA-mCherry

Size: 1679 bp

Vector: pSB1C3; both are chloramphenicol resistant

Cloning Sites: EcoRI/Spel/XbaI/PstI/

Applications of BBa_K2650001

In early transformations, this part is visibly red/pink on plates and is therefore assumed to be correctly expressing and folding the mCherry portion of the fusion protein. Further application notes are pending. First 2 pictures below show the transformation of BslA mCherry in Bacillus (labeled B). The last 2 pictures show the transformation of BslA mCherry in E.coli (petri dish H).

T--OLS_Canmore_Canada--Trans_B.jpeg T--OLS_Canmore_Canada--Trans_B_UV.jpeg T--OLS_Canmore_Canada--Trans.jpeg T--OLS_Canmore_Canada--Trans-UV.jpeg

Refrences

Bio Basic, Lisa Oberding

User Reviews

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